碩士 / 國立臺灣大學 / 動物學研究研究所 / 93 / We cloned a novel inhibitor of apoptosis protein (IAP) family member, PmIAP, from Penaeus Monodon. PmIAP contains three baculoviral IAP repeat (BIR) domains followed by a RING finger domain. The BIR and RING finger domains of PmIAP share striking amino acid sequence similarity with those of cIAP1, cIAP2, XIAP, and DIAP2, suggesting evolutionary conservation. Pm-IAP was 4.7 kb long, including a coding region of 2097 bp preceded by 633 bp (5'' untranslated region) and followed by 2039 bp (3'' untranslated region). Pm-IAP encoded a protein (Pm-IAP) of 698 amino acids with a predicted molecular mass of 76.9 kDa. RT-PCR analysis revealed that the steady-state level of PmIAP mRNA is highest in lymphoid organ among examined tissues, including hepatopancrease, stomach, hemocyte, gill, nerve, heart and muscle. Furthermore, the PmIAP RNA level in LO cells was not changed significantly after WSSV infection, although LO cells undergo apoptosis upon viral infection. The immunofluorescence staining of Spodoptera frugiperda Sf-9 cells transiently expressing PmIAP revealed that the PmIAP was mainly localized at cytoplasm of Sf-9 cells. To demonstrate the anti-apoptosis activity of Pm-IAP, we cotransfected the Drosophila reaper and Pm-IAP gene into Sf-9 cells. The result showed that Pm-IAP can block apoptosis in Sf-9 cells induced by Drosophila Reaper.
| Identifer | oai:union.ndltd.org:TW/093NTU05312016 |
| Date | January 2005 |
| Creators | Yu-Chen Kuo, 郭昱辰 |
| Contributors | , 郭光雄, 羅竹芳 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 64 |
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