碩士 / 國立臺灣大學 / 動物學研究研究所 / 93 / Nervous Necrosis Virus（NNV）can induce acute infection and mass mortality of grouper fry and larva. The aim of this study is to detect and analysis of the differentially expressed grouper genes after NNV infection. By suppression subtractive hybridization (SSH) and Northern analysis, two cDNA sequences（FB2, FB9） were selected by forward subtraction and two cDNA sequences（R1H1, R2H12）were selected by reverse subtraction. These four cDNA sequences were analyzed by Blast-n program, and the results revealed that FB2 is similar to the gene of alpha-microglobulin/HI-30 precursor, FB9 is similar to the gene of glyceraldehyde 3-phosphate dehydrogenase (G3PDH), R1H1 is similar to the gene of beta globin, and R2H12 is similar to the gene of liver-basic fatty acid binding protein (Lb-FABP). The expression of either FB2 and FB9 increased significantly in the moribund fish 9 days after NNV infection. On the other hand, the expression of R1H1 and R2H12 decreased drastically in the fish showing clinical syndrome of NNV disease in the early stage of NNV infection.
| Identifer | oai:union.ndltd.org:TW/093NTU05312020 |
| Date | January 2005 |
| Creators | Li-Ling Huang, 黃麗玲 |
| Contributors | Shau-Chi Chi, 齊肖琪 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 73 |
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