博士 / 國立臺灣大學 / 漁業科學研究所 / 93 / Expression of gnrh3 neurons started in the olfactory pit at 24-26 hours post-fertilization (hpf) and migrated into forebrain region during early larvae stage. We generated transgenic zebrafish expressing green fluorescent protein (GFP) and LacZ driven by the gnrh3 promoter. In gnrh3-transgenic fish, the expression of GFP and LacZ were similar to gnrh in the olfactory placode, olfactory bulb and telencephalon. To understand the function of GnRH at gnrh3 neuron development, we have done the morpholino knockdown and over-expression analysis. Reduced GFP and gnrh3 cell numbers, and delayed migration were caused by gnrh3 morpholino knockdown. The gnrh2 can rescue the morphants partially. Over-expression of gnrh3 can induce the earlier development of gnrh3 neurons. The gnrh3 expression was not in cyc, oep and smu mutant. PKI misexpression can rescue the gnrh3 defect in oep mutant. PKA and PKI misexpression caused the GFP cell numbers decrease and GFP ectopic expression, respectively. The normal gnrh3 expression and PKA pathway were involved in the development of gnrh3-expressing neurons during embryos. Transgenic zebrafish with specific gnrh3-GFP-LacZ expression in the brain offers a genetic tool to study the origin and migration of gnrh3 neuron lineage.
| Identifer | oai:union.ndltd.org:TW/093NTU05451012 |
| Date | January 2005 |
| Creators | Ming-Wei Kuo, 郭明偉 |
| Contributors | 羅秀婉 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 128 |
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