碩士 / 大仁科技大學 / 環境管理研究所 / 93 / According to data collected from monitoring stations in lakes and reservoirs maintained by the Environmental Protection Administration (EPA), the problem of eutrophication has escalated in recent years. The phenomenon of eutrophication gives rise to algae proliferation. Due to its interference with the quality of treated water, and the operation of water treatment facilities, the development of technologies for the removal of algae growth has been the focus of many research papers for the past several years, both domestically as well as internationally, and less attention has been paid the study of characteristics of algae through spectral analysis.
The fluorescence meter, due to its non-destructive nature of measurement, makes it feasible to quality the difference in structural characteristics between that of suspended particles and dissolved organic matter. Due to the lack of research carried out on the study of the fluorescent characteristics of algae, and the correlation of characteristics between dissolved organic matter and algae in the eutrophic lake, the use of the fluorescent meter EEFM (Excitation-Emission Fluoresence Matrix) to compare this relationship is worth considering. In this study, in addition to the carrying out of spectral analysis, the THMFP (Trihalomethane formation potential) from different kinds of algae will also be described.
There is an indication that algal population in three eutropic lakes had a positive correlation with temperature, presence of chlorophyll, level of total phosphorus and turbidity. The percentage of the dominant algae, Chlorococcaceae and Chroococcaceae were determined to be 90 % in CCL and FS reservoirs. In MD reservoir, the value was decreased to 70 % in the first and second sampling, but Nitzschiaceae and Coscinodiscaceae made up 72%, and were found to be the dominant algal species in the third and fourth sampling.
Also, different autofluorescent colors and intensity were observed when different kinds of algae were placed under an epifluoresent microscope. Melosira granulate (Eherenberg) Ralfs Var. granulate and Pediastrum simplex Meyen Var. simplex as an example, it was revealed that the intensity of algal autofluorescence and coloration upon the addition of Lugol''s solution was weakened as compared with the adding of no Lugol''s solution.
Two samples each were taken from CCL Lake and FS Lake, and it was found that the percentages of carbon content of different MWCO (molecular weight cut-off) in dissolved organic matter (DOM) were also varied as the percentage of algal species were changed. This suggests that the increase in Chlorococcaceae will lead to an increase in the percentage of carbon content of dissolved organic matter which the MWCOs is higher than 5K. Furthermore, it reveals that a decrease in Chroococcaceae will lead to a decrease in the percentage of carbon content of dissolved organic matter (DOM) which the MWCOs is less than 5K. However, during the first and second sampling at MD reservoir, the variation in the percentage of carbon content of dissolved organic matter were opposite from those from CCL and FS reservoirs. This effect may be attributed to the decrease in the percentage of Coscinodiscaceae among the total algal population, and an increase of Cosmarium in total algal population.
The THMFP value in the water source as obtained from CCL, FS and MD reservoirs, as compared with that of the filtered water source, indicates that the former value was larger than latter. Furthermore, Chlorococcaceae, Coscinodiscaceae, Cosmarium and Nitzschiaceae do not favorably form THMFP. On the other hand, Chroococcaceae, Hydrodictyaceae, and Oscillatoriaceae could form well THMFP.
The peak values of EEFM of the water source, filtrates and extracted algae obtained from CCL and FS reservoirs were always found to be within the range of excitation/emission wavelength (EX (nm)/EM(nm)), 220~230/300~310,230/330~350, and 270~280/330~340. However, the peak value of excitation/emission wavelength of 240~250/300~310 can be found in the third and fourth water samples taken from MD reservoir. This may be that the dominant algal species, Chlorococcaceae and Chroococcaceae, which appeared in CCL and FS reservoirs in March and April, were different from Nitzschiaceae and Coscinodiscaceae, which appeared in MD reservoir in November and December.
| Identifer | oai:union.ndltd.org:TW/093TAJ05700020 |
| Date | January 2006 |
| Creators | Zhi-Wen Zhang, 張志文 |
| Contributors | Wen-Liang Lai, 賴文亮 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 83 |
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