碩士 / 東海大學 / 環境科學系 / 93 / Abstract
In this thesis, an amount (10 mg) of dried fish sample was placed in a 7-mL teflon microreaction vessel. Two-stages of microwave digestion (500 μL of nitric acid and then 100 μL of hydrogen peroxide) were performed at 85℃ for 10 min. After cooling to room temperature, the pH of the acidic fish mixture was adjusted to 5 - 6 by NaOH. Appropriate amounts of 2,3-dimercaptopropane-1-sulfonate (DMPS, 500 μL) and sodium acetate buffer (pH=6.0, 1 mmol) were added to the mixture to form a mercury-DMPS complex. The complex was preconcentrated on two home-made C18 cartridges in series, and then each cartridge was eluted with methanol and adjusted to 0.50 mL. A portion (50 μL) was introduced into a graphite cuvette and then measured by GFAAS (λ=253.7 nm). The peak heights in absorbance were used for a quantitative analysis.
The observed values [270 ± 4 (ng/g), 3370 ± 20 (ng/g), and 4600 ± 110 (ng/g)] of total Hg in three fish certified reference materials (NRC TORT-2, NRC DOLT-3, NRC DORM-2) were in good agreement with the certified values [270 ± 60 (ng/g), 3370 ± 140 (ng/g), and 4640 ± 260 (ng/g) Hg, respectively ]. The method detection limit (MDL, 3σ) for Hg was found to be 22 (ng/g); the calibration graph was linear up to 1350 (ng/g). Recoveries of 96.7-102.2% were obtained by spiking known amounts of mercury to the three CRM fish samples.
| Identifer | oai:union.ndltd.org:TW/093THU00518007 |
| Date | January 2005 |
| Creators | Lin, Fang-yi, 林芳儀 |
| Contributors | Kuo, Mao-sung, 郭茂松 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 89 |
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