碩士 / 國立陽明大學 / 生醫光電工程研究所 / 93 / Micro-contact printing by using photo lithography techniques is well used in cell engineering for cell manipulation. We try to utilize micro-contact printing technology to study the interaction between ECM and cell migration. We demonstrate that linear pattern can be successfully generated and printed with fibronectin on cover glass. Fish keratocytes were used as our cell model system to study cell migration on printed pattern. Cell migration was recorded by time-lapsed imaging system and further analyzed by velocity. In micro-contact printing result, we found cells were reshaped by the patterns and cell adhesion was limited in fibronectin pattern shape by IRM and phase contrast microscopy observation. Focal adhesion molecules also accumulate along linear pattern edge. Migrating path of fish keratocyte cells were followed the printed micron pattern, and the migrating velocity was also enhanced. The increasing velocity results two different groups which can identify from statistic results, which can not be found in homogeneous substrate. We conclude that printed linear micron pattern with fibronectin affect cell migration both in path guiding and increased velocity. In sub-micro-contact printing result, we found the cell sense the sub micro pattern by focal adhesion molecules accumulation on patterns, IRM image associate with pattern. But migrating distance were decrease, and migrating orientate variety. Calcium oscillation of the migrating fish keratocyte appears to correlate with the motility kinetics of cell. We believe that using micro-contact printing is useful to study the interaction between ECM and cell migration.
| Identifer | oai:union.ndltd.org:TW/093YM005114009 |
| Date | January 2005 |
| Creators | Yun-Cin Luo, 羅韻晴 |
| Contributors | Chi-Hung Lin, 林奇宏 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | en_US |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 46 |
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