碩士 / 國立陽明大學 / 解剖暨細胞生物學研究所 / 93 / Heatstroke-induced neuronal damage can result from the heat and lower perfusion of cerebral blood flow. We investigate the morphological changes of neurons and astroglia in the CA3 and CA4 sectors of hippocampus and dentate gyrus in heatstroke-induced rats by suing immunocytochemistry, electron microscopy and Western blotting. In addition, we also evaluate the effects of heat preconditioning and glycerol injection on the neurons of the hippocampus. Our results indicate that the duration of the heatstroke induction is 99.8±8.3 minutes. A striking characteristic is that there appear the symptom of hyperthermia and hypotension in the heatstroke-induced rats. Moreover, we find that the reactive pyramidal neurons of CA3 and CA4 sectors show intensely methylene blue staining in association with the hypertrophyied cytoplasmic processes of the astroglia. Furthermore, the granule cells of the dentate gyrus show high tolerance effects in response to the heatstroke. The heat precondition may cause to activate the different signaling pathways via astroglia to mitigate the reactive cell changes of neurons. On the other hand, administration of glycerol can increase the survival time after the onset of heatstroke. Furthermore, we also show that the reactive astroglia contain the dilation of perikaryon and the hypertrophied processes. Such morphological features may be involved in the homeostasis of the perivascular space of the astroglia and the blood brain barrier of the blood vessels. In conclusion, our data suggest that the astroglia may play an important role in the protection of neurons against the stress and insult from the environmental toxicity, especially in the synapses of pyramidal neurons with granule cells of hippocampus.
| Identifer | oai:union.ndltd.org:TW/093YM005391003 |
| Date | January 2005 |
| Creators | Wen-Hua Han, 韓文華 |
| Contributors | Shang-Ming Yu, 游祥明 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 89 |
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