碩士 / 元培科學技術學院 / 生物技術研究所 / 93 / Proline-rich tyrosine kinase 2 (PYK2) is a member of the focal adhesion kinase (FAK) family of non-receptor protein tyrosine kinase. FAK family is renowned by its relationship to cell adhesion machinery. Human PYK2 has been shown to be sensitive to the change of intracellular calcium concentration and stress signals. The zebrafish (Danio rerio) has recently emerged as an important model organism for studies of embryonic development, vertebrate genetics and human disease. Advantages of using zebrafish are external fertilization, large brood size, the optical clarity and large size of its embryos and rapid development. To elucidate the role of pyk2 during embryogenesis, we had initiated a project of using zebrafish as our model. Whole-mount in situ hybridization was performed to analyze the developmental expression of pyk2 gene during zebrafish embryogenesis. The results showed that the transcripts were detected ubiquitously from fertilization through segmentation stage. From 24-hr post-fertilization onward, pyk2 transcripts can also be found in many but not all tissues. Most obviously, elevated signals were detected at the ventral site of developing brain. However, skin, pronephric ducts and blood island also showed high level of pyk2 expression. In the trunk, only slight expression of pyk2 gene can be detected in somite and spinal cord, while no expression can be found at the notochord. To study the function of pyk2 gene during zebrafish embryogenesis, the anti-sense morpholino (MO) was injected into the zebrafish embryo to inhibit the translation of pyk2 mRNA. The pyk2-MO-injected embryos showed some defects in the developing brain and cardiovascular system. In addition, kinked tails were also observed in these morphants. Using the zebrafish as animal model, we demonstrated that pyk2 gene is involved in the development of brain and cardiovascular system.
| Identifer | oai:union.ndltd.org:TW/093YUST7108004 |
| Date | January 2005 |
| Creators | Chi-Hsuan Cheng, 鄭啟軒 |
| Contributors | Yu-Jen Chen, Jen-Ning Tsai, 陳有任, 蔡振寧 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 64 |
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