碩士 / 嘉南藥理科技大學 / 生物科技系暨研究所 / 94 / Morinda citrifolia, commonly known as noni, is reported to have antibacterial, antiviral, antioxidant and anti-tumor activity. The aim of this study is to partition the ethanolic extracts of the noni fruits, brown stems and leaves with n-hexane, 1-butanol and ethyl acetate, which these fractions will be used to investigate the anti-EBV lytic proteins-Rta, Zta, EA-D expression, and cell-toxic effect,the relationship between antiviral activity and antioxidant compounds and anti-EBV mechanism. Our results showed that the ethyl acetate fractions prepared from fruits has the highest total phenolic concentration, but the highest DPPH scavenging activity are the 1-butanol fraction. Moreover, the expression of EBV lytic proteins is inhibited by 100 mg/ml of ethyl acetate extract. The 1-butanol fractions of brown stems and leaves, which exhibit the high total phenolic contents and DPPH scavenging activity, inhibit the expression of Rta, Zta and EA-D at a concentration of 200 mg/ml. We also found that an fraction prepared from noni fruit with 1-butanol at 500 mg/ml inhibits the expression of the ATF-2. The fraction also display a stronger inhibitory activies than that of quercetin on the transcription of BZLF1 and BRLF1. The results suggest that the antioxidant compounds present in Morinda citrifolia plant may contribute to the anti-EBV activies.
| Identifer | oai:union.ndltd.org:TW/094CNUP5111030 |
| Date | January 2006 |
| Creators | Chu-Yun Cheng, 鄭筑勻 |
| Contributors | none, none, 林翠品, 陳師瑩 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 76 |
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