碩士 / 嘉南藥理科技大學 / 環境工程與科學系暨研究所 / 94 / This study focused on the isolation of attached diatoms taken from Szu-Tsao Wildlife Reserve and their tolerance of the heavy metals copper and cadmium. The results showed that the attached diatoms could be isolated from the nature and pure culture by the Marine enrichment medium f/2 medium under conditions of 30℃, pH8 and light intensity 100μmol．photons．m-2．S-1. Seven species were identified under the light microscope (LM) and scanning electric microscope(SEM). Three genera with six species were under order Pennales and one species was under order Centrals. The isolated diatoms were strain STSP1(Amphora sp1), STSP2(Amphora sp2), STSP3(order Centrales), STSP4(Navicula sp1)、STSP5(Navicula sp2)、STSP6(Navicula sp3) and STSP7(Nitzschia sp1).
Furthermore, the heavy metal copper and cadmium were added to the pure culture diatoms of strain STSP1 and STSP2 separately and cell growth of the diatoms were observed. We found that after 57 hours, strain STSP1 and STSP2 would grow in 0.6ppm and 0.9ppm concentration of copper separately. On contrast, strain STSP1 and STSP2 could grow as well in solution containing cadmium in concentration as high as 6ppm, however, a concentration of 8ppm cadmium ions could damage of them.
In addition, growing recovery culture revealed that strain STSP1 and STSP2 could tolerate very high concentration of copper. Both of them could survive in copper ion solution with a concentration of 0.9 to 9ppm. In conclusion, we believe that strain STSP1 and STSP2 could act as bioindicators in environment polluted by the heavy metals copper and cadmium。
| Identifer | oai:union.ndltd.org:TW/094CNUP5515019 |
| Date | January 2006 |
| Creators | Chan-Ting Yeh, 葉展廷 |
| Contributors | Suen-Zone Lee, 李孫榮 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 142 |
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