碩士 / 中山醫學大學 / 生化暨生物科技研究所 / 94 / To date, it is well accepted that Regulator of G-protein signalling（RGS） proteins terminate signalling by accelerating the intrinsic G alpha-GTPase activity and recycling the G-protein complex back to its inactive GDP-bound heterotrimeric configuration. The RGS protein can be divided into 15 subfamilies（RGS1~RGS15） in accordance with the structure and the biological function. The RGS domain, a conserved 120-aa region exists in all RGS proteins, functions as a negative regulator through interacting with activated Gα subunit and turn off the signaling. In addition, RGS proteins contain other domains that can associate with other protein to enhance the G-protein signaling. In this study, we attempted to investigat the expression pattern and function of RGS7 in zebrafish during development. Using RT-PCR analysis, RGS7 was expressed from fertilization to 96 hours post-fertilization of Zebrafish. Expression of RGS7 was found in brain、aorta ventralis、intermediate cell mass and somite by whole-mount in situ hybridization. Microinjection of RGS7-specific antisense morpholino oligonucleotide into fertilized zebrafish embryos leaded to interfere normal heart development and the heart function. Phenotype such as weakness of heart beat, swollen pericardial cavity and abnormal heart tube formation was found in morphants. Taken together, there results suggest that RGS7 protein plays an important role in the early development of the zebrafish heart.
| Identifer | oai:union.ndltd.org:TW/094CSMU5107030 |
| Date | January 2006 |
| Creators | Chien-Hao, 黃建豪 |
| Contributors | 許立松 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 79 |
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