碩士 / 中華醫事學院 / 生物科技研究所 / 93 / Vibrio parahaemolyticus is one of the most important food-borne pathogens in Taiwan , Japan and other coastal countries. In Taiwan , this pathogen occupy more than 70% by the bacterial food -poisoning at the first occurring annually.
In this study, the traditional culture and polymerase chain reaction were employed based on five sets of primers (toxR、tlh、tdh、trh、toxRS) to analyze sample form environment , and establish distribution of Vibrio parahaemolyticus on southwest sea area in Taiwan and epidemiology database. We were collected seawater and organic material from coastal areas of southwestern Taiwan.to detect at experiment period. The total of V. parahaemolyticus was determined using PCR amplification of the toxR、 tlh genes ,however the pathogenic V.parahaemolyticus numbers was determined by using PCR amplification of the tdh、 trh、 toxRS genes. The study results showed traditional culture was only detected toxR(45.8%) , and the PCR can detected toxR(79.6%) and tlh (89.8%). As for distribution of the V. parahaemolyticus pathogenicity gene from various samples were detected tdh (5.1%)、 trh (9.3%) ,and toxRS (80.6%) ,respectively. In addition, we were isolated V. parahaemolyticus from environment, only 1 trh gene, occupy 0.5% in total bacteriun. If compare V. parahaemolyticus numbers from different sample types found organic samples greater than water samples. In the case of V.parahaemolyticus numbers isolated from three sampling site: Anping＞Hsing-Da＞Chien-Chen. The serum type experiment showed V.parahaemolyticus was the most serotype O2. in Taiwan coast.
| Identifer | oai:union.ndltd.org:TW/094HWAI7111010 |
| Date | January 2006 |
| Creators | Nian-Qun Chen, 陳念群 |
| Contributors | Fu-Lin Chang, 張福林 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 0 |
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