碩士 / 國立成功大學 / 工程科學系碩博士班 / 94 / In this paper the manipulation of Ca-alginate microspheres, using a microfluidic chip, for the encapsulation of gold nanoparticles with internal and external gelation methods is presented. Our strategy is based on hydrodynamic-focusing on the forming of a series of self-assembling sphere structures, the so-called water-in-oil (W/O) emulsions, in the cross-junction microchannel.
In the internal gelation method, these fine emulsions, consisting of 1.5% w/v Na-alginate and 1.0% w/v calcium carbonate, are then dripped into an oil solution containing 20% v/v glacial acetic acid and 1% v/v Tween 80 to accomplish Ca-alginate microspheres in an efficient manner. The mechanism is that acetic acid reacts with the calcium carbonate to release the calcium ions, and these calcium ions are then crosslinking with the Na-alginate to produce Ca-alginate microspheres.
In the external gelation method, these fine emulsions, consisting of aqueous Na-alginates, are then dripped into a solution of 20% calcium salt to accomplish Ca-alginate microspheres in an efficient manner.
We have demonstrated that one can control the size of chitosan microparticles from 100 µm to 1000 µm in diameter (with a variation less than 10%) by altering the relative sheath/sample flow rate ratio The microfluidic chip is capable of generating relatively uniform micro-droplets and has the advantages of active control of droplet diameter, simple and low cost process, and high throughput.
| Identifer | oai:union.ndltd.org:TW/094NCKU5028051 |
| Date | January 2006 |
| Creators | Tzung-Heng Lai, 賴宗亨 |
| Contributors | Yu-Cheng Lin, 林裕城 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 84 |
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