碩士 / 國立成功大學 / 化學工程學系碩博士班 / 94 / Nannochloropsis oculata is an oleaginous microalga. It can fix the carbon dioxide, transforming it into cellular lipid through photosynthesis. With its characteristic of abundance in lipid, it can be an excellent source of biodiesel since the intracellular lipid can be easily extracted and converted to biodiesel. In order to get large lipid production from microalgae, the design of optimum cultivation strategy is very important.
In this study, we propose a novel two-step cultivation strategy of microalgae: In the first step, the cultivation was carried out in a circulation type photobioreactor with sufficient nitrogen source. In the second step, the cultivation was carried out in a flat-plat photobioreactor with limiting nitrogen source. Circulation type photobioreactor was composed of a rectangular flat plate and a medium reservoir at the bottom. It was characterized by enhanced illumination area. It could improve the shortcomings of low illumination area, incomplete mixing in the traditional open pond. We obtained a large amount of microalgae through this step. Flat-plate photobioreactor was characterized by high irradiance per working volume. Microalgae could accumulate lipid in the nitrogen limiting environment. We obtained high lipid content of microalgae through this step.
In the study, we separately investigated the optimum culture conditions for the first step and second step cultivation. We also compared our two-step cultivation strategy with the traditional single step cultivation methods, i.e. batch culture in the flat-plate photobioreactor or in the circulation type photobioreactor. In the two-step strategy, the final lipid yield was about two-folds of that in the flat-plate photobioreactor or six-folds of that in the circulation type photobioreactor. The results proved that the two-step cultivation strategy could enhance the lipid yield effectively.
| Identifer | oai:union.ndltd.org:TW/094NCKU5063048 |
| Date | January 2006 |
| Creators | Jun-Liang Ye, 葉俊良 |
| Contributors | Wen-Teng Wu, 吳文騰 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 80 |
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