碩士 / 國立中央大學 / 生命科學研究所 / 94 / The study tried to establish a 3T3-L1 preadipocyte culture and differentiation system for the screening of inhibiting agents of lipid accumulation in algal natural products. Inoculated preadipocytes were growing in 24-well plate of FBS-DMEM medium. After reaching confluent, culture medium were added insulin, dexamethasone and 3 isobutyl-1-mehtyl-xathine, and refreshed with new the medium containing insulin every two days afterwards. This culture system used 100 �嵱 genistein (27�n�慊/ml) as positive control in comparison with the MeOH extracts of the algal cells including NP01, NP02, NP03, CR02, FE01, FE02, and FE03 of blue green algae, Ha01, Gc01, Grs04 and Pd01 of red algae, and Ec01 of brown algae. Concentrations of the algal extract applied for the screening were 27, 270 and 2700�n�慊/ml. Lipid contents in the differentiated cells were analyzed by Oil red O staining. All the algal extracts tested were found to be of no effect like genistein in inhibition of cell differentiation and lipid accumulation. It was also found that the algal extracts showed no cytotoxicity or growth inhibition to the cells, except FE02 that showed slight growth inhibition to the cells but no cytotoxicity. Although we did not find any inhibition activity of lipid accumulation in the algal extracts, we found this culture and differentiable system of 3T3-L1 cell line were reliable and practicable for the screening of anti-obesity natural products.
| Identifer | oai:union.ndltd.org:TW/094NCU05105002 |
| Date | January 2005 |
| Creators | Hsiu-Ju Hung, 洪綉茹 |
| Contributors | Hong-Nong Chou, 周宏農 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 70 |
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