碩士 / 國立嘉義大學 / 水產生物學系研究所 / 94 / Abstract
The aim of this study approaches the feasibility of using microalgae to uptake NH4+ in water. There are four parts in this study: the first is to approach the effect of temperature on growth rate and NH4+-uptake rate of four microalgae; the second is to approach the effect of culture media, algal growth stages and inoculated algal concentrations of algae on the growth of Tetraselmis chui; the third is to approach the effect of light-path length of algal incubator, light intensity, aerial intensity and convention rate on growth rate and NH4+-uptake rate of Tetraselmis chui; the fourth is to approach the feasibility of the reuse of pond water whose NH4+ is uptake by microalgae, then filtrated by hard clam.
The temperature for maximal growth rate and NH4+-uptake rate of Isochorysis, Tetraselmis, and Nannochloropsis is 25℃, while 35℃ is for Chaetoceros. At 25℃, the daily biomass productions per liter of Isochrysis, Nannochloropsis, Tetraselmis and Chaetoceros are 0.043,0.037,0.041 and 0.085 g dry weight, respectively. Daily NH4+-uptake per liter of these algae is 2.99, 4.27, 4.26 and 9.41 mg NH4+-N, respectively. Therefore, Tetraselmis has the highest ability in NH4+-uptake among these four microalgae.
Inoculation with different growth stages of Tetraselmis chui affects their nutritional salt requirement. The addition of minor elements affects growth rate of Tetraselmis chui in mid-logarithmic phase insignificantly, while they increase growth rate of the algae in early-stationary phase. The addition of NH4+ increases the growth rate of algae in mid-logarithmic phase significantly. During 5-day culture, pHs in culture with NH4+ and NO3- as nitrogen sources are decreased and unchanged, respectively. Inoculated concentrations of algae affect growth rate and production of Tetraselmis chui in mid-logarithmic phase. The growth rate of algae with inoculated concentrations of 8 and 16 ×104 cells/ml reaches the highest. However, the production of algae with inoculated concentrations of 16 and 41 ×104 cells/ml is maximal. The growth rate and NH4+-uptake of Tetraselmis chui in the condition of light-path length of 8㎝, light intensity of 240 μE/m2.s and aerial intensity of 7 L/min are reach the highest in this experiment. They are 1.55 per day and 7.9 mg NH4+-N /L.day, respectively.
In the growth experiment of tilapia, the control group (1) only proceeds one week due to a high ammonia-N in the water, while the control group (2) and the treatment group proceed three weeks. No significant difference in the growth rate and FCR of tilapia, and in ammonia-N in the water between the control group (2) and the treatment group was found. This indicates that the water exchange could be replaced by the reused water treated with algae and hard clam. In addition to saving of 340 L of artificial sea water, 340 L of algae water (OD750 nm 0.35) for clam food is produced in this study.
| Identifer | oai:union.ndltd.org:TW/094NCYU5613009 |
| Date | January 2006 |
| Creators | Shih-Yuan Chen, 陳士元 |
| Contributors | An-Chin Lee, 李安進 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 88 |
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