Taxonomic studies of crab’s megalopae from Hsiang-Shan wetland--- mtDNA COI sequence as identification tool / 新竹香山溼地沿岸蟹類大眼幼體之分類研究--以COI基因核酸序列為輔助鑑定工具

碩士 / 國立新竹教育大學 / 人資處應用科學系教學碩士班 / 94 / From March 2004 to September 2005, adult crabs and their megalopae were collected from the Hsiang-Shan wetland. I use mtDNA COI sequence as genetic marker to identify different species of megalopae. According to the sequence alignment, I have 22 species of megalopae belong to 6 families. There are 1) Portunidae:Portunus pelagicus, Charybdis annulata, Thalamita spinimana; 2) Xanthidae:Leptodius sanguineus; 3) Mictyridae:Mictyris brevidactylus; 4) Ocypodidae:Ocypode stimpsoni, Uca arcuata, Uca vocans borealis, Uca lactea lactea, Scopimera longidactyla, Scopimera bitympana, Macrophthalmus banzai; 5) Grapsidae: Metopograpsus thukuhar, Gaetice depressus, Helice formosensis, Helice subquadrata, Metaplax elegans, Parasesarma plicatum, Parasesarma pictum, Perisesarma bidens, Chiromantes haematocheir; 6) Gecarcinidae:Discoplax hirtipes. For the 22 species of megaplopae, I established a diagnostic key for each species and detail illustrations of morphological characters were given.
In addition, after my studies the crab records of Hsiang-Shan wetland are 54 species, Ozius rugulosus, Eriphia sebana, Pinnotheres sinensis, Pinnotheres haiyangensis, and Helice subquadrata are new records. For the crab fauna of 54 species, I established mtDNA COI nucleotide sequences of 41 species belong to 10 families. For the megalopae identification by mtDNA sequence, excluded 22 knowing species, 16 species were unidentified due to lack of adult sequence comparison. More crabs collects in the future will get more mtDNA sequence data, and more megalopae will be identified.

Identiferoai:union.ndltd.org:TW/094NHCT5115012
Date January 2005
CreatorsHuang Shu-Chen, 黃淑珍
Contributors楊樹森
Source SetsNational Digital Library of Theses and Dissertations in Taiwan
Languagezh-TW
Detected LanguageEnglish
Type學位論文 ; thesis
Format133

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