碩士 / 國立屏東科技大學 / 熱帶農業暨國際合作研究所 / 94 / This study was to determine the effects of noradrenaline on the immunity and physiological factors of the freshwater giant prawn, Macrobrachium rosenbergii and analyzed in 0, 2, 4, 8 and 16 hrs after injecting the prawn with noradrenaline at 0.5, 5.0, and 50.0 pmol or saline per prawn. The effects of noradrenaline were determined by measuring the total hemocyte count (THC), different hemocyte count (DHC), phenoloxidase activity, respiratory burst, superoxide dismutase activity, phagocytic activity and clearance efficiency susceptibility, hemolymph glucose and lactate contents, osmotic and ionic regulation, oxyhemocyanin and the base-acid balance. The results show that semigranular cell, phenoloxidase activity, respiratory burst, superoxide dismutase activity, phagocytic activity and clearance efficiency were significantly decreased during 2 and 8 hrs. The hyaline cell was significantly increased at 2 hrs. No significant differences were observed in THC and granular cell. All of the immune parameters returned to the values same as the control at 16 hrs after receiving noradrenaline, except hyaline cell. Injection of noradrenaline also significantly increased the mortality of M. rosenbergii challenged with L. garvieae. These results suggest that stress-inducing noradrenaline suppresses the immune system, which in turn promotes the susceptibility of M. rosenbergii to L. garvieae. In the parameter of physiological response, hemolymph glucose and lactate contents increased significantly; Na+, K+, osmolality, oxyhemocyanin significantly decreased; hemolymph pCO2, HCO3- and TCO2 increased significantly were observed. No significant differences were observed in pH value, total protein level, chloride ion contents and pO2. These results indicated that noradrenaline either reduce the immunological resistance of giant freshwater prawn or affect the physiological responses.
| Identifer | oai:union.ndltd.org:TW/094NPUST643029 |
| Date | January 2006 |
| Creators | Hong Ming Di, 洪明迪 |
| Contributors | Cheng, Winton, 鄭文騰 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 86 |
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