碩士 / 國立清華大學 / 分子與細胞生物研究所 / 94 / Partial or complete genomic duplications have occurred several times during the evolution of vertebrates, resulting in several duplicated genes derived from a common ancestral gene. Ionotropic glutamate receptors (iGluRs) mediate excitatory synaptic transmission in the vertebrate central nervous system. The ionotropic glutamate receptors are classified into three major subtypes, the AMPA-, kainite-, and NMDA-preferring receptors. Zebrafish expresses two duplicated GluR2 genes, GluR2α and GluR2β, as well as two duplicated NR1 genes, NR1.1 and NR1.2. Duplication-degeneration complementation (DDC) is a model hypothesized to preserve duplicated genes. If DDC is involved in the preservation of duplicated genes, the expression patterns of the paralogous genes are expected to be different and compensatory. In this thesis, whole-mount in situ hybridization was employed to study the expression patterns of GluR2 and NR1 duplicated genes. Experimental results showed that the expression of GluR2β increased significantly in the retina of zebrafish between 2 to 3 day postfertilization; however the expression of GluR2α in the retina increased only mildly over the same peroid. The expression of GluR2β in the telencephalon, tectum opticum and corpus cerebelli are higher than that of GluR2α. The expressions of NR1.1 and NR1.2 are complementary in the regions of telencephalon, corpus cerebelli and lobus caudalis cerebelli. These results showed that DDC may be involved in the preservation of duplicated genes of GluR2 and NR1 during evolution.
| Identifer | oai:union.ndltd.org:TW/094NTHU5061022 |
| Creators | Gong-Her Wu, 吳恭和 |
| Contributors | Wei-Yuan Chow, 周姽嫄 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 33 |
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