碩士 / 國立清華大學 / 生物科技研究所 / 94 / Besides high mortal rate to cultured shrimp, White Spot Syndrome Virus (WSSV) also causes high mortality to other species of aquatic crustacean. As a result, there is an urgent need to find the solutions for epidemic of WSSV. From previous studies demonstrate that VP28, an envelop protein of WSSV, is critical for WSSV infection; and the infection of WSSV could be neutralized or attenuated through the existence of anti-VP28 antibodies within shrimp body. However, the concentration of anti-VP28 antibodies within shrimp must have to maintain a stable and efficacious level for inhibition of WSSV infection. Actually, PEGylated protein can sustain more stable concentration within the body of vertebrate organism and appeared to be more efficacious of its biological functions. The specific aims of this study is tried to maintain the concentration and duration of anti-VP28 antibodies within shrimp body through PEGylation modification. After PEGyaltion of anti-VP28 antibodies (aVP28), antibodies were introduced into shrimp bodies through either intramuscular injection or oral feeding. From the results, PEGylaton provided aVP28 twice times of duration, as comparing to non-PEGylated. In fact, this result is the first report that monoclonal antibodies could extend the duration within the body of invertebrate organism through PEGylation modification. As oral feeding, neither PEGylated nor non-PEGylated aVP28 could not survive from the GI track. Overall, the aVP28 might be a practical treatment for inhibition of WSSV infection after PEGylation.
| Identifer | oai:union.ndltd.org:TW/094NTHU5111011 |
| Date | January 2006 |
| Creators | Jung-Ta Lu, 呂榮達 |
| Contributors | Yiu-Kay Lai, Chi-Min Chen, 黎耀基, 陳啟銘 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | en_US |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 52 |
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