碩士 / 國立清華大學 / 生物資訊與結構生物研究所 / 94 / After heating chlorella pyrenoidosa at 46.5℃ for an hour, and culturing them under light or in the dark regime, we found that there are quite difference between chlorella’ pattern and physiology on result. The cell under light regime is etiolating and the cell under dark regime is kept green.
We used the DAPI and Acridine orange to observed the cell by Confocal Microscopy, we can see the DNA of the cell in light regime were condensed seriously but the cell under dark regime is not seriously. About the pattern of the chlorophyll; the chlorophyll of the cell in the light regime almost disappear in 10 hours, on the other hand the chlorophyll of the cell in the dark regime existence to the end but no photosynthesis activities.
Other two fluorescence stains, SYBR Green 1 and SYTOX Green are also used to analyze the difference under between light regime and dark regime. Evans blued and Trypan blue to compare how the rupture situations of cell membrane were. Here we found out the number of rupture chlorella in light regime is much higher than dark regime, also the percentage of rupture cell more than 60%.
According to result we obtained, we know the light could cause injured of the chlorella and no matter in light regime or dark regime, the DNA won’t dissolution and disappear.
We think that the physiology mechanism worth studying deeply in chlorella of the situation in the future.
| Identifer | oai:union.ndltd.org:TW/094NTHU5112008 |
| Date | January 2006 |
| Creators | 張晉嘉 |
| Contributors | 徐邦達 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 57 |
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