碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 94 / The paper deals with the effects of sulfide on the immune response of white shrimp Litopenaeus vannamei and Kuruma shrimp Marsupenaeus japonicus and their susceptibities to Vibrio alginolyticus when the shrimp were exposed to various sulfide concentrations. The immune parameters included THC (total haemocyte count), hyaline cell, granular cell, semi-granular cell, phenoloxidase activity, respiratory burst (release of superoxide anion), superoxide dismutase (SOD), phagocytic activity and clearance efficiency to V. alginolyticus.
L. vannamei held in 35 ‰ seawater were challenged with V. alginolyticus at a dose of 6.26 ×105 colony-forming units (cfu) shrimp-1, and then placed in water containing different concentrations of sulfide at 0 (control), 49, 105, 488 and 967 μg l-1. Mortality of shrimp exposed to 488 and 967 μg l-1 sulfide was significantly higher that of other treatments after 24-96 h. In another experiment, L. vannamei exposed to 488 and 967 μg l-1 sulfide for 6-48 h showed decreased hyaline cell, THC, phenoloxidase activity, phagocytic activity and clearance efficiency, but increased respiratory burst and superoxide dismutase activity. However, no significant differences in semi-granular cell and granular cell were observed. The release of superoxide anion by L. vannamei exposed to 488 and 967 μg l-1 sulfide was considered to be cytotoxic to the host.
M. japonicus held in 35 ‰ seawater were challenged with V. alginolyticus at a dose of 2.7 ×106 colony-forming units (cfu) shrimp-1, and then placed in water containing different concentrations of sulfide at 0 (control), 56, 112, 525 and 1076 μg l-1. Mortality of shrimp exposed to 56 μg l-1 sulfide was significantly higher than that of other treatments after 24-144 h. In another experiment, M. japonicus exposed to 525 and 1076 μg l-1 sulfide for 24 h showed decreased hyaline cell, THC, phenoloxidase activity, phagocytic activity and clearance efficiency, but increased respiratory burst and superoxide dismutase activity. No significant difference in semi-granular cell and granular cell were observed. The release of superoxide anion by M. japonicus exposed to 1076 μg l-1 sulfide was considered to be cytotoxic to the host.
In conclusion, sulfide increased the susceptibility of L. vannamei and M. japonicus against V. alginolyticus infection by a depression in immune ability including decreases in hyaline cell, THC, phenoloxidase activity, phagocytic activity and clearance efficiency.
| Identifer | oai:union.ndltd.org:TW/094NTOU5086001 |
| Date | January 2005 |
| Creators | Sz-Wei Hsu, 許思微 |
| Contributors | Chen Jiann-Chu, 陳建初 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 78 |
Page generated in 0.0015 seconds