碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 94 / Abstract
The objectives of the this study were to investigate the possible roles of corticotropin-releasing hormone (CRH), corticotropin-releasing hormone receptor (CRH-R1) and Interleukin-1β (IL-1β) in the gill of black porgy. We designed acute salinity change, cortisol oral administration and in vitro gill culture experiments to study CRH, CRH-R1 and IL-1β gene expressions in gill and hypothalamus. The quantitative PCR measurement of the respective genes was developed to measure those genes contents.
In this study, when fish was transferred from seawater (SW) to fresh water (FW) and also from FW to SW, CRH mRNA expression was increased in gill in day 1 and day 4. CRH-R1 mRNA expression in gill was only increased from FW to SW, but there was no effect from SW to FW in both day 1 and day 4. IL-1β increased from SW to FW in day 1 and day 4, but there was no effect from FW to SW in day 1 and decreased in day 4. Acute salinity change affected CRH, CRH-R1 and IL-1β gene expressions in gill. In the time course experiment, the increase of CRH-R1 was found in gill. CRH-R1 was decreased in gill after handling stress. Cortisol had a negative feedback effect on CRH and CRH-R1 in gill, but there was no effect in hypothalamus.
In in vitro gill culture, FW gill had a higher IL-1β expression than SW gill. In contrast, SW gill had higher Na+/K+-ATPase transcripts than FW gill. In in vitro gill culture, CRH and cortisol had no significant effects on CRH, CRH-R1, IL-1β and Na+/K+-ATPase transcripts except cortisol effects in gill CRH-R1.
| Identifer | oai:union.ndltd.org:TW/094NTOU5086015 |
| Date | January 2006 |
| Creators | Yi-Hui Chen, 陳怡慧 |
| Contributors | Ching-Fong Chang, 張清風 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 88 |
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