碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 94 / Abstract
Sea anemone fish is so popular species in the aquarium fish of sea water all the time. In order to reduce catch and replace with wild individual, reduce the exhaustion of the natural resources, establishing the artificial reproduction technology of sea anemone fish has already become an important subject.
Newly hatched tomato anemone fish( Amphiprion frenatus) larvae has high mortality and low growth rate at the period of ingesting rotifer( Brachionus plicatilis). The experiment was divided into three parts. The first experiment was to observe the growth of the embryo and to record the larvae grow to maturity of tomato anemone fish in order to understand the time of spawn hatch and edible change for fish larvae. The experimental result was finding the fertilized eggs would development completed and hatched in the night of seventh day after spawned. Newly hatched larvae could ingest rotifer immediately, and then the larvae could be fed Artemia in forth day.
The second experiment was feeding rotifer at density 1, 5, 10 rotifer/ml three days to the larvae of the tomato anemone fish to affirm the appropriate feeding density of rotifer for fish larvae. The experimental result was that the larvae of tomato anemone fishes had concurrently good survival and growth rate in feeding rotifer at density 5 rotifer/ml.
The third experiment used four species of microalgae(Chaetocero muelleri., Isochrysis galbana., Nannochloropsis oculata., Tetraselmis chui) and bread yeast ( Saccharomyces spp.) to enrich rotifer respectively, and then fed it separately the larvae. Using this method is affirmed the appropriate nourishment to rotifer for tomato anemone fishes. The experimental result showed that feeding rotifer that enriched with Nannochloropsis oculata could be had concurrently survival and growth rate of tomato anemone fish larvae.
Comprehensive our experimental results, the newly hatched tomato anemone fish larvae can ingest rotifer at once, and take for three days. Feeding the rotifer at density 5 rotifer/ml enrich with Nannochloropsis oculata could be improved survival and growth rate during this period. Using this method to feed tomato anemone fish larvae can reach 80 ％ of survival rate and increase 40 ％ of growth rate, which as an outstanding achievement in breeding tomato anemone fish in a large amount.
| Identifer | oai:union.ndltd.org:TW/094NTOU5086037 |
| Date | January 2006 |
| Creators | Sheng-Wei Chien, 錢昇威 |
| Contributors | Fan-Hua Nan, 冉繁華 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 78 |
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