碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 94 / The standard microbiological method used for aquatic environment cholera detection can hardly be adapted in environment background test for its no economic benefit. However, it would result in omission in epidemic control. Due to its cultivation difficulty, the bacterial number is often underestimated and it will result in discrimination difficulty on pathogenicity. The main purpose of this study is to establish a simple, effective and fast detection method for cultivation environment. In this study, there are 173 farm samples from 10 counties. Samples are divided into two groups on particle size. There are 346 samples in total which 315 are effective and 31 are non effective. Two samples of Vibrio cholerae O1 are positive in Yunlin and Kaohsiung respectively. Two samples of Vibrio cholerae O139 are also positive in Yunlin and Pingtung. ctx gene of V. cholera had not been found. The four farms with V. cholera are all marine cultures. In standard cassical microbiological methods, the whole procedure takes at least 3 to 4 days. Using polymerase chain reaction method, it takes just only 3 to 4 hours, and will not limited by viable but non-culturable (VBNC). According to the difficulty to obtain living strains for detection limit test,the V. carchariae and V. harveyi are used for simulation of detection limit test. In Trypticase soy broth, the detection limit of V. carchariae is 110CFU/ml and 2.7×103CFU/ml for V. harveyi, but it will rise to 106CFU/ml in sea water. It indicates that sea water will interfere the process of polymerase chain reaction.
| Identifer | oai:union.ndltd.org:TW/094NTOU5086039 |
| Date | January 2006 |
| Creators | Chiu-Chun Chen, 陳丘錞 |
| Contributors | Cheng-Hui Lin, 林正輝 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 39 |
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