碩士 / 國立臺灣海洋大學 / 食品科學系 / 94 / A cellulase-producing bacterium, could form a clear zone on carboxy methyl cellulose (CMC) agar plates and designated as C-6, was isolated from soil and identified to be a Cellulomonas sp.. The optimal conditions for its growth were pH 6.8 in nutrient broth with 1.0 % carboxy methyl cellulose (CMC) at temperature of 30 oC and 150 rpm of shaking speed. The maximal cellulase activity was obtained after 96 hr incubation at 30 oC. In order to hydrolyze the cell wall of Chlorella, 10 % and 20 % extracellular enzyme solution hydrolyzed 10 % and 20 % Chlorella water were tested. Higher chlorophyll and protein contents, and lower residual dry weight were observed after the hydrolysis with 10 % extracellular fluid hydrolyzed 20 % Chlorella water. Although the cellulase activity lost gradually during 2 hr hydrolysis at 50 oC, breaking of Chlorella cell walls and release of chlorophyll were clearly observed after 30 min hydrolysis. According to the microscopic observation and measurement of chlorophyll content, obvious lysis of cell walls was obtained after 60 min hydrolysis. In order to increase Chlorella nutritive value, the extracellular fluid was determined protease activity and this property can hydrolyze protein from breaking of Chlorella. After, the peptide contents raised gradually and the free amino acid contents also raised during 3 hr hydrolysis.
| Identifer | oai:union.ndltd.org:TW/094NTOU5253024 |
| Date | January 2006 |
| Creators | Xian-Hui Peng, 彭顯惠 |
| Contributors | , 江善宗, 殷儷容 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 76 |
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