博士 / 國立臺灣大學 / 動物學研究所 / 94 / In order to simplify the survey of pesticide (including carbamate, organochlorine, organophosphate and synthetic pyrethroid pesticides) residues in fishery products (including bivalve, crustacean, fish and cuttlefish), multiresidue determination methods were developed through a solid phase extraction (SPE) technique. In the present procedures, samples were extracted with acetonitrile except for bivalve and cuttlefish samples. Mixed solvent of water, acetone and acetonitrile was added to bivalve prior to homogenization, whereas water was needed in cuttlefish samples for blending. For both kinds of sample, an additional procedure of salting-out was needed during extraction. Tandem SPE cartridges of C18 and aminopropyl, using acetonitrile as the only solvent, were used to clean up extracts from either method. A total of 91 pesticides in four major pesticide groups were tested in this study. Gas chromatography (GC, equipped with electron capture detector and flame photometric detector) and high-performance liquid-chromatography (HPLC) equipped with fluorescence detector were used for analysis. The validation of the method was evaluated for each fishery product using samples spiked with all pesticide standards at three concentration levels. The results indicated percentage of recovery ranged from 60% - 120% and coefficients of variation < 20% for all but 10 of the pesticides analyzed (including 1-naphthol, 3-hydroxy carbofuran, aldicarb sulfoxide, heptachlor, trifuralin, acephate, dichlorvs, methamidophos, monocrotophos and omethoate). Residue extraction techniques described in this report are rapid and suitable for screening of pesticide residues in monitoring programs.
For assessment of consumer’s exposure to pesticide residues in fisheries products, the methos described above were used. The fisheries products in this study comprise fish, bivalve, crustacean and cephalopod collected from different types of market in Taiwan between the years of 2001-2003. A total of ninety-one pesticide residues belonging to four major pesticide groups were tested and analyzed. The test results show that 65.40% of fish, 93.55% of shellfish, 84.92% of crustacean and 98.33% of cephalopod samples contain no detectable residues. There are only two kinds (organochlorine and organophosphate) of totally six pesticides (DDTs, dieldrin, chlorpyrifos, fenitrotion, fenthion and prothion) that have been detected from the fisheries products in this study. For there were pesticides present in the fish products, consumption of fisheries product there was no zero risk. But the exposure of consumer did not exceed the acceptable daily intakes (ADI). For male, there was the highest risk in exposure to dieldrin, which the percentage of ADI was 93.56%. This study also suggests that a yearly monitoring program for organophosphate pesticide residues in fish is necessity.
| Identifer | oai:union.ndltd.org:TW/094NTU00312031 |
| Date | January 2006 |
| Creators | Feei Sun, 孫 斐 |
| Contributors | Shiu-Nan Chen, 陳秀男 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 171 |
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