碩士 / 國立臺灣大學 / 海洋研究所 / 94 / Phycocyanin, one of the major biliproteins from blue green algae, has various biological and pharmacological properties. It is a water-soluble and non-toxic fluorescent protein pigment. The present study used two-step column chromatographic method to isolate and purify c-phycocyanin from a marine Synechococcus. The isolation involved a multi-step treatments of the crude extract by fractional precipitation with ammonium sulfate, then by Q-sepharose column and finally ceramic hydroxyapatite column, giving a purity ration A620/A280 of 4.9. The yield of c-phycocyanin was 35% from Synechococcus dried powder. Respectively, further the purity and homogeneity were confirmed by native and SDS-PAGE electrophoresis. The estimated molecular weight of the phycocyanin was 128 kDa as determined by Sephadex S-200 gel filtration and electrophoresis.
Phycocyanin from the reported alga was assayed in vitro for proliferation stimulation in murine macrophages and for anti-tumor activity against human mammary and pancreas tumor cells, using MTT method. The results indicated that macrophage proliferation increased by 38% while treated with phycocyanin at the concentration of 65 μg/ml and the stimulation activity was a dose-dependent manner. Tumor cells used in the present study reduced markedly in growth rate in the presence of phycocyanin, i.e. reduced by 80% at concentration of 65 μg/ml. The obtained phycocyanin was also an antioxidant as determined by DPPH method. Its antioxidation activity, as expressed in % scavenge of oxygen radicals, reached 36% at concentration of 78 μg/ml. The above findings revealed that marine Synechococcus and the extracted phycocyanin can be a potent source for biomedical research, as well as therapeutic agent not only in immune stimulation and modulation, but also in tumor growth inhibition.
| Identifer | oai:union.ndltd.org:TW/094NTU05274048 |
| Date | January 2006 |
| Creators | Hui-Chun Hsu, 徐慧君 |
| Contributors | 黃穰 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 85 |
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