碩士 / 國立臺灣大學 / 漁業科學研究所 / 94 / This purpose of this study is aimed to know the effect of dietary β-glucan on non-specfic immune response in small abalone (Haliotis diversicolor supertexta) and use sodium alginate with β-glucan to observe survival and attachment of small abalone post-larvae.
In experiment 1 to compare agar (1%)+Spirulina powder (24.5%), agar (1%)+blue-green algae powder (24.5%), agar (1%)+Chlorella powder (24.5%), agar (1%)+Gracilaria powder (24.5%), agar (1%)+Ulva powder (24.5%),agar (1%)+Sodium alginate (24.5%),agar (1%),Sodium alginate (24.5%) to induce ingestion agents. The best treatment is Sodium alginate (24.5%).
In experiment 2 to compare the control (without β-glucan), 2, 4, 8, 16 g/kg β-glucan and blank (Gracilaria sp.) by observing the THC (total haemocyte counts), O2-, PR (phagocytic rate), PI (phagocytic index), PO (phenoloxidase), GOT (glutamate oxalacetate transaminase), GPT (glutamate pyruvate transaminase), luminol- and lucigenin- enhanced chemiluminescence. The result showed that dietary β-glucan can enhance THC, O2-, PR, PI, luminol- and lucigenin- enhanced chemiluminescence. In all treatments showed that no significant differences in SOD, GOT and GPT. That means β-glucan can enhance non-specfic immune responses in small abalone. The treatment in 4g/kg β-glucan is the best effect on O2- activity(0.563±0.004) and PR(0.199±0.014%).
In experiment 3 to compare sodium alginate and 4g/kg β-glucan by adding into agar (1%) to observe attachment number and survival. The result showed that sodium alginate can increase post-larvae of small abalone attachment number(219.66±9.07). This treatment of 4g/kg β-glucan add into agar (1%) can enhance post-larvae of small abalone survival on plate during 15 days.
| Identifer | oai:union.ndltd.org:TW/094NTU05451019 |
| Date | January 2006 |
| Creators | Jeng-Tzyo Shie, 謝政佐 |
| Contributors | 陳秀男 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 93 |
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