碩士 / 國立高雄大學 / 生物科技研究所 / 94 / The most difference between fish and mammalian central nervous system (CNS) is the fish produce new neurons throughout their lifespan. Therefore, it is suggested fish CNS is a well material to study CNS development. A cell line designated TB2 has been established from tilapia brain and has been subcultured more than 100 times. In this study, TB2 cells were characterized by using immunocytochemistry, Western blot and RT-PCR methods to verify if they have neuronal and glial characters.
The immunocytochemistry results showed TB2 cells express GABA, astrocyte markers glial fibrillary acidic protein (GFAP), vimentin, S100, oligodendrocyte marker galactocere- broside (GalC) and oligodendrocyte precursor marker (A2B5). Most of the cells were identified express GFAP (63％), vimentin (71％) and S100 (61％). GABA (12％), A2B5 (12％) and GalC (14％) were observed in some of the cells. Western blot analysis also verified TB2 cells can express tyrosine hydroxylase (TH), GFAP, vimentin, glutamine synthetase (GS), S100 and myelin basic protein (MBP) and the molecular weights are similar to those brain tissue. The RT-PCR results showed TB2 cells derived from tilapia brain consider that TB2 cells and tilapia brain have the same GFAP sequence. In addition, cytokeratin 8 that has been reported in nerve tissue was also demonstrated appear in TB2 cells by RT-PCR.
Since TB2 cells were identified coexpress the glia-specific and neuron-specific molecular markers, it is suggested TB2 cells might be the neural stem cells than can produce neurons, astrocytes and oligotentrocytes in the cultures. However, as no neural stem cell specific marker was evidence in TB2 cells, more studies are need to verify.
| Identifer | oai:union.ndltd.org:TW/094NUK05111006 |
| Date | January 2006 |
| Creators | Wen-Chieh Chen, 陳文傑 |
| Contributors | none, 溫秋明 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 82 |
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