碩士 / 長庚大學 / 生化與生醫工程研究所 / 95 / In this study we examined the behavior of freshly isolated rat hepatocytes cultivated within alginate and galactose-modified alginate gel beads. The hydrogel scaffold can mimic the in vivo extracellular matrix and provide a three-dimensional environment for cell proliferation and differentiation. Alginate modified with galactosamine, which provides a galactose moiety for recognition by hepatocytes surface receptor, was found to drastically up-regulate the metabolic functions of hepatocytes. Hepatocytes encapsulated in alginate beads were cultured in a pack-bed reactor and medium re-circulating speed in reactor was found to influence cell aggregate size, albumin secretion rates, and urea production rates.
| Identifer | oai:union.ndltd.org:TW/095CGU00723016 |
| Date | January 2007 |
| Creators | Sheng-Ying Wang, 王圣瑩 |
| Contributors | Jyh-Ping Chen, 陳志平 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 111 |
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