碩士 / 嘉南藥理科技大學 / 生物科技系暨研究所 / 95 / White spot syndrome virus (WSSV) is a widespread viral agent in shrimps and other crustaceans population. It has caused severe mortalities and huge enconomic losses to the shrimp farming industry, not only in Asia but also globally. Sequence analysis revealed a low level of homology between most WSSV ORFs and known genes from GeneBank, and this virus has been a member of the genus Whispovirus within a new virus family called Nimaviridae.
This study is to investigate the function of one open reading frame (designated wssv162) from the WSSV genome of Taiwan isolate. We try to construct the recombinant plasmids containing the ORFs, and to express them by transformation into Escherichia coli, BL-21(DE3). Until now, the 6x His-tagged fusion proteins containing the WSSV162 were expressed successfully and used to prepare a specific antibody for western blotting. On the other hand, we transfected EGFP-WSSV162 construct into insect cells, and examined the cellular localization of the WSSV162 protein. The numbers of transfected insect cells, which expressed EGFP-WSSV162 fusion proteins, are low. But, it is interesting that EGFP-WSSV162 fusion proteins are mainly expressed in the cytoplasma of transfected insect cells. Then we also found that some EGFP-WSSV162 fusion proteins seem to be expressed as inclusion bodies.
In order to study the interactions between WSSV162 protein and host shrimp proteins. We used the pull-down assay to analyze the possibilities of ineteractions between His6-WSSV162 fusion proteins from E. coli and shrimp proteins.
| Identifer | oai:union.ndltd.org:TW/095CNUP5111010 |
| Date | January 2007 |
| Creators | Pei-Chein Hsieh, 謝佩倩 |
| Contributors | Nai-Yueh Tien, 田乃月 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 81 |
Page generated in 0.0921 seconds