碩士 / 中山醫學大學 / 營養學研究所 / 95 / Based on the data mining against NCBI（National Center for Biotechnology Information） data band. The analysis found a fatty acid desaturase-2 (FADS2) of human（NP_004256/NM_00426）and zebrafish（NP_571720.2/NM_131645）.
The FADS2 cDNA of zebrafish were successfully amplified with a continuous open reading frame of 1335 bp encoding a protein of 444 amino acid. But the FADS2 cDNA of human were not successfully amplified with a continuous open reading frame of 1335 bp .The FADS2 of human and zebrafish were with a calculated molecular mass of 52.27 and 52.14 kDa .
Zebrafish FADS2 were cloned and expressed with a 6-histidine tag for specific purification. The purified recombinant protein have mobility of 52.14 kDa on sodium dodecyl sulfate(SDS)-polyacrylamide gel electrophoresis. We discovered that E. coli expression system may not be an appropriate system to express stable abundance of the recombinant protein。
In addition, Northern blotting analysis showed that fatty acid desaturase-2 is expressed in the Sprague-Dawley rat tissues, such as brain, heart, liver, stomach, and testis.
| Identifer | oai:union.ndltd.org:TW/095CSMU5513006 |
| Date | January 2007 |
| Creators | Hsiao-Chun, 吳曉君 |
| Contributors | 劉德中 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 133 |
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