碩士 / 大葉大學 / 分子生物科技學系碩士班 / 95 / White spot syndrome virus (WSSV) is a highly pathogenic and prevalent virus affecting crustacean. Disease caused by WSSV is the greatest challenge to word wide shrimp aquaculture. In this stuffy, full length cDNA of Agrocybe aegerita lectin (AAL) gene was cloned, recombinant AAL (rAAL) was expressed by prokaryotic (Escherichia coli) expression system and the activities of this recombinant protein including the anti WSSV effects were analyzed. Amino acids analysis showed that AAL with a carbohydrate recognition domain (CRD) and protein modification motif. AAL might be with strong agglutination activities for lacking cystine in its amino acids composition. Native gel electrophoresis demonstrated the rAAL with the ability for self-forming a dimmer structure. In agglutination test, rAAL agglutinated rabbit Red Blood Cell (RBC) at very low concentration (0.25ng/ml). The anti-WSSV assays performed on Litopenaeus vannamei showed an obvious effect. The rAAL treated shrimp groups were with greater than 20％ survival rate than the untreated ones in five days.
| Identifer | oai:union.ndltd.org:TW/095DYU00061011 |
| Date | January 2007 |
| Creators | Kun-Hung Chang, 張琨鴻 |
| Contributors | Yun-Shiang Chang, 張雲祥 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 87 |
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