碩士 / 輔仁大學 / 生命科學系碩士班 / 95 / In this study, we intend to isolate microcystin from the mass culture of Microcystis aeruginosa. To establish the microcystis mass culture, we need to scale up the culture from the size of stock culture, 2L flask to 400L glass tank, using enriched media, irradiation and aeration. It was found the increase of culture vessel had lower stationary phase all density. We speculate it was due to the light limitation grow cell density and the length of light path. This is a need of a well-designed photobioreactor and automatic process to maintain the highest growth rate with sufficient irradiation. It was also found there were various amount of toxin released in the medium at different growth phase of microcystis. Through the experiment of the possible effect of inoculant ratio on the growth rate and toxin releasing, we were able to confirm the previous data of toxin releasing was due to the integration of dead cell.
Regarding the toxin isolation, we compared various solvent of different composition for extraction and determined that 70％ methanol extraction had a batter extraction rate of microcystin and better result than before in combination with a modified partition operation followed. After the extraction of aqueous methanol extract solution with dichloromethane more non-toxin components can be removed. Through the application of Diaion SP825 adsorbent resin, target microcystin could be concentrated from water layer. The application of LH-20 in the separation of microcystin, using 20％ methanol elution can recover most of the MC-LR and reach a purity of 61％ in the collected fraction, and final separation using a reverse phase high performance liquid chromatography could have the result of above 95％ purity product. It is possible to increase the recovery rate of microcystin from the extract of microcystis biomass and reduce the cost of microcystin production.
| Identifer | oai:union.ndltd.org:TW/095FJU00105004 |
| Date | January 2007 |
| Creators | Cheng-Hong Hsieh, 謝政宏 |
| Contributors | Hong-Nong Chou, Jyh-Yih Leu, 周宏農, 呂誌翼 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 55 |
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