碩士 / 國立中興大學 / 生命科學院碩士在職專班 / 95 / The main purpose of this experiment is to explore the antioxidant activities of three herbs’ extracts, Houttuynia cordata, Saussurea involucrate, Liu-Dou-Huang and to evaluate the possibilities of formulating these herbs being part of anticancer formula in Chinese medicine. Methanol and double deionized water at 4℃, 25℃, and 50℃, respectively, were used as solvents to extract the components of these herbs. The extracts were used to measure antioxidant activity, such as DPPH scavenging ability and reducing power. Extracts from green tea were used as comparison. The total phenolic contents of extracts were measured in order to reveal the relationship between the antioxidant activity and the total phenolic contents.
The DPPH antioxidant activity of methanol extract of Houttuynia cordata is better than those of water extracts. The water extracts from different temperatures did not show obvious differences in their antioxidant activity. But, the reducing power of the 50℃ extract of Houttuynia cordata is better than those of 25℃, 4℃, and methanol extract. The DPPH antioxidant activity of water extract of Saussurea involucrate is better than that of Liu-Dou-Huang. However, the latter performs better than the former one in its reducing power. In as low density as 1mg/mL, green tea does have 73% in DPPH antioxidant activity, and 0.9 in reducing power as well. The reason for green tea performs as the best of all the samples may be related with its richness in phenolic contents. Total phenolic contents and DPPH scavenging ability showed high relationship (r=0.88), only mildly related with reducing power (r=0.54). Nonetheless, the amount of phenolic contents of other extracts from these three main research targets did not show any relative with DPPH scavenging ability and reducing power. It is obvious that other components other than phenolic compounds also play the important role on antioxidant activity to protect the living organisms.
We are certain that the extracts from Houttuynia cordata, Liu-Dou-Huang, green tea and Saussurea involucrate all prove good antioxidant activities. Further researches and evidences on cell protection of antioxidant activity are needed to possible application these herbs into anticancer formula.
| Identifer | oai:union.ndltd.org:TW/095NCHU5105033 |
| Date | January 2007 |
| Creators | Hsueh-Ping Peng, 彭雪萍 |
| Contributors | Jung-Yie Kao, 高振益 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 49 |
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