Optimization of production of astaxanthin and fructooligosaccharides by yeast Xanthophyllomyces dendrorhous / 利用酵母菌Xanthophyllomycesdendrorhous同步生產蝦紅素及寡果糖最適化條件之研究

碩士 / 中興大學 / 食品暨應用生物科技學系 / 95 / Astaxanthin, one kind of carotenoids, has been used as a pigment source in the poultry and aquaculture industries to improve the red color of products. Due to the color and antioxidative activity, astaxanthin can also be used as food colorant and antioxidant in medicine. In Japan, astaxanthin had been produced as anticancer agents.
Fructooligosaccharides (FOS) are widely found in natural plants and are also synthesized from sucrose by transfructosylation of microbial β-fructofuranosidase or fructosyltransferase. They are not only beneficial to food process but also non-digestible by human enzyme. FOS can stimulate the beneficial bacteria and Bifidobacterium in intestines, thus to improve the health of human and animal.
The total carotenoid content of X. dendrorhous BCRC-21346 and the high-astaxanthin producing mutant mutant NCHU-FS601 were 286 and 1,128μ g g-1 yeast, respectively. Both strains had the ability for co-production of astaxanthin and fructooligosaccharides which neokestose was the major fructooligosaccharide. In flask, the maximum volumetric of total FOS from sucrose of fermentation in yeast malt (YM) medium with 200 g L-1 sucrose by X. dendrorhous BCRC-21346 and X. dendrorhous NCHU-FS601 were 128.60 g L-1 and 53.00 g L-1, respectively. In YM medium with 400 g L -1 sucrose, the maximum volumetric of total FOS produced by X. dendrorhous BCRC-21346 and X. dendrorhous NCHU-FS601 were 296.40 g L-1 and 102.90 g L-1, respectively. In 1.5 L fermenter, the maximum volumetric of total FOS produced by X. dendrorhous BCRC-21346 in YM medium with 200 g L-1 sucrose and 400 g L-1 sucrose were 127.78 g L-1及295.02 g L-1. The maximum productivity in the same condition in fermenter were 15.97 g L-1 h-1及19.67 g L-1 h-1, which higher than in flask.

Identiferoai:union.ndltd.org:TW/095NCHU5255056
CreatorsYi-Ming Tai, 戴宜銘
Contributors方繼
Source SetsNational Digital Library of Theses and Dissertations in Taiwan
Languagezh-TW
Detected LanguageEnglish
Type學位論文 ; thesis
Format66

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