碩士 / 國立成功大學 / 生理學研究所 / 95 / Results from epidemiological studies suggest that regular exercise improves cognitive function. Animal studies have shown that exercise increases levels of various neurotrophic factors and enhances learning and memory behavioral performance. Integrin-associated protein (IAP) expression is associated with memory formation in rats. Nuclear respiratory factor 1 (NRF-1), a transcription factor for IAP, can increase neurite outgrowth, which is mediated, at least in part, by upregulating its downstream IAP gene. Whether NRF-1 and IAP are involved in exercise-facilitated learning and memory capacity is unclear. Therefore, we used male BALB/c (3-month-old) mice as an animal model to answer this question. Mice were randomly divided into exercise and control groups. Animals in the exercise group received 4 weeks of treadmill exercise training and sacrificed at different time course after the last run. IAP protein expression and distribution were determined by Western blotting and immunohistochemistry, respectively. Whether exercise activates NRF-1 to upregulate IAP gene expression was clarified by electrophoresis mobility shift assay (EMSA). Our results demonstrated that after chronic exercise training, 1) one-trial inhibitory avoidance learning performance was improved; 2) the hippocampal IAP protein level was increased after last run of chronic exercise; and 3) no significant difference in NRF-1 binding activity was found after last run 0h and 1h of 4-wk exercise training. These results suggest that chronic exercise training could improve learning and memory in PA and enhance IAP expression in hippocampus, but not through activate NRF-1.
| Identifer | oai:union.ndltd.org:TW/095NCKU5116009 |
| Date | January 2007 |
| Creators | Shiang-yun Chen, 陳湘芸 |
| Contributors | Hsiun-ing Chen, 陳洵瑛 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 57 |
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