碩士 / 國立成功大學 / 環境工程學系碩博士班 / 95 / This research mainly combined with the field investigation to estimate the availability of assimilation
of nutrients by Spirogyra. The results could further apply to decrease the concentrations of nitrogen
and phosphorous and improve the eutrophication in the reservoir or watershed.
The results of primary experiments indicated the assimilated amounts of nutrients increased when the pH
of medium was 9 without stirring. It was probably attributed to the flocculated effects of Spirogyra.
The period of culture was 4 days and the highest assimilated amounts of carbonate, nitrate and
phosphorous were 12%, 70% and 25%, respectively.
The results of batch experiments showed the effects of total nitrogen and ortho-phosphate per dry
weight of Spirogyra with various environmental factors. The assimilated amounts by Spirogyra would
better when the pH, temperature and concentration of nutrients were classified to high levels. The
highest assimilated amounts of nitrogen and ortho-phosphate per dry weight of Spirogyra were 36.89 mg/g
．L and 6.99 mg/g．L. After transferring and analyzing with ANOVA software, we could find there was no
significant effect existed on the assimilation of total nitrogen. But the temperature and
concentrations of phosphate-adding were the major factors and had the apparent effects for assimilating
by Spirogyra.
To compare the results of assimilating amounts of total nitrogen we found that the values increased in
some experiments after cultivating. It was referred to the organic nitrogen released from algae via
metabolism function to induce the variations of total nitrogen to become negative and the
concentrations per dry weight of Spirogyra were about 0-1.36 mg/g．L. The total lipid contents
increased with increasing the assimilated amounts of nutrients and the highest value of raising was
77.35%. It was implied the Spirogyra could apply to decrease the concentrations of nitrogen and
phosphorous in the reservoir and watershed and also had the potential for developing bioresource.
We also used the molecular biology technique to identify the test algae and the results indicated the
S1, S2 and S3 could categorized as Spirogyra geuns and Zygnemataceae family. The different sampling
zones of watershed didn’t discover the variations in the types of attached algaes.
| Identifer | oai:union.ndltd.org:TW/095NCKU5515029 |
| Date | January 2007 |
| Creators | Yi-chun Kuo, 郭意均 |
| Contributors | Ming-muh Kao, 高銘木 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 98 |
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