碩士 / 國立嘉義大學 / 農學研究所 / 95 / Adventitious shoots were successfully regenerated using leaf or petiole of explants from Rieger Begonia ( Begonia × hiemalis ‘Barkos’ ). Leaf or petiole sections were cultured on MS medium containing different concentration of NAA ( 0.1、1 mg/l ) and BA ( 0.1、0.5、1、5 mg/l ). The culture medium was optimized for the leaf or petiole explants derived from shoot which was supplemented on MS medium with 1 mg/l NAA and 0.5 mg/l BA and observed 8.5 or 12.6 shoots from each explant after eight weeks, respectively. Hundred percent shoot regeneration and 8.4 shoots per explant was obtained when leaf cutting position near the petiole position of leaf explants and it was significantly different from leaf cutting position near the middle and margin of explants. Histological studies showed that adventitious shoots originated from both leaf or petiole epidermis layer and then differentiate to the formation of apical meristems and leaf primordial structures and it did not show intact somatic embryogenesis. Histological observation revealed that regeneration of leaf or petiole explants via organogenesis. Further elongation of adventitious shoots was obtained from each explant on full strength MS solid medium or liquid- shake medium with average of 10.9 and 12.2 plantlets, respectively. However, shoot only a few plantlets was obtained shoot of petiole explants on full strength MS solid medium or liquid-shake medium. All the regenerated plantlets were 90 % survival rate and continued to grow after the transfer to plastic pots. No morphological aberrations were observed in the regenerated plants.
| Identifer | oai:union.ndltd.org:TW/095NCYU5416015 |
| Date | January 2007 |
| Creators | Ming-Chi Shu, 許銘志 |
| Contributors | Cherng-Kang Liao, 廖成康 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 0 |
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