碩士 / 國立嘉義大學 / 獸醫學系研究所 / 95 / This study detect Nocardia seriolae by using polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP). Eighty isolates of N. seriolae from cultured fish was identified, together with a type strain N. seriolae BCRC 13745, were characterized by Sau-PCR. In addition, minimal inhibition concentrations (MICs) and inhibition zone diameter for ampicillin, amoxicillin, doxycycline, erythromycin, florfenicol, lincomycin and oxytetracycline were determined for each isolates based on Mueller–Hinton agar. Susceptibility data were tabulated in scattergram and analyzed by error rate bounded method. Both PCR and LAMP can used for rapid detection of N. seriolae with specificity, but the LAMP method was more sensitive than PCR. Data generated by Sau-PCR fingerprinting could be differentiated in 6 pattern types. There were 8~12 fragments in each pattern type. The resistance breakpoints for doxycycline (MIC≧16 μg/mL), erythromycin (MIC≧4 μg/mL), florfenicol (MIC≧32 μg/mL), lincomycin (MIC≧8 μg/mL), oxytetracycline (MIC≧16 μg/mL) were developed except ampicillin and amoxicillin. In conclusion, Sau-PCR analysis can be used to discriminate N. seriolae isolates obtained from different habitat. The breakpoints are applicable to detect antimicrobial resistance of N. seriolae from cultured fish.
| Identifer | oai:union.ndltd.org:TW/095NCYU5541002 |
| Date | January 2007 |
| Creators | Wei-Lung Kao, 高維隆 |
| Contributors | Jiann-Hsiung Wang, 王建雄 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 0 |
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