碩士 / 國立嘉義大學 / 水產生物學系研究所 / 95 / The present study was to examine the immune responses of hard clam (Meretrix lusoria, 37 ± 2 mm shell length) that fed different diets of microalage（Chaetoceros muelleri, Tetraselmis chui and mixture of C. muelleri + T. chui or unfed treatments）at different temperatures.
Clams that had been acclimated to different temperatures (20℃、25℃or 30℃), were examined for the immune parameters（morphology of haemocytes, total haemocyte counts, different haemocyte counts, phenoloxidase activity, respiratory burst, superoxide dismutase activity and phagocytic activity against pathogen Vibrio alginolyticus）.
The haemocytes of M. lusoria were divided into three types: (1) hyalinocytes: with none granule in the cytoplasm, (2) small eosinophilic granulocytes and (3) large eosinophilic granulocytes. The total haemocyte counts, hyalinocyte counts, small eosinophilic granulocyte counts and large eosinophilic granulocyte counts of clams acclimated to 20℃ were significantly lower than those of clams acclimated to 25℃ and 30℃. The phenoloxidase activity of M. lusoria acclimated to 25℃ was significantly higher than that of clams acclimated to 20℃ or 30℃. The respiratory burst, SOD activity and phagocytic activity against V. alginolyticus significantly increased with the temperature.
After 7 and 14 days exposure to 20℃, the total haemocyte counts, hyalinocyte counts, small eosinophilic granulocyte counts, respiratory burst, SOD activity and phagocytic activity against pathogen V. alginolyticus in clams that fed microalage C. muelleri, T. chui and mixture of C. muelleri + T. chui or unfed treatment were significantly lower than those of clams reared at 25℃ or 30℃. When clams exposed to 20℃ for 7 and 14 days, the clearance efficiency of clam against pathogen V. alginolyticus fed C. muelleri, mixture or unfed treatments were significantly lower than that of clams exposed to 25℃ or 30℃. In comparison with unfed treatments, the clearance efficiency of clams fed T. chui significantly increased to 46% and 55%, respectively, for the clams exposed to 25℃ or 30℃ for 7 days. After 14 days exposure to 20℃、25℃ or 30℃, the clearance efficiency of clams fed T. chui were significantly increased to 28% ~ 34%.
The total haemocyte counts and hyalinocyte counts of clams fed T. chui significantly increased after 7 days exposure to 20℃. The phenoloxidase activity, respiratory burst and SOD activity of clams fed T. chui also significantly increased after 7 and 14 days exposure to 20℃. The clearance efficiency of M. lusoria fed T. chui for 14 days significantly increased to 32%, and higher than clams that fed C. muelleri, mixture or unfed treaments. Clams held in 25℃, the total haemocyte counts, small eosinophilic granulocyte counts and respiratory burst of M. lusoria fed T. chui for 7 days are significantly higher than unfed treaments. The clearance efficiency of M. lusoria fed T. chui significantly increased to 46%, after 7 days exposure to 25℃. After 14 days, the clearance efficiency of M. lusoria fed C. muelleri, T. chui or mixture significantly increased to 25%、26％ and 21％, respectively. When clam held in 30℃, no significant difference on the total haemocyte counts, small eosinophilic granulocyte counts, SOD activity and phagocytic activity were observed for the clams that fed with different microalage diets or unfed treatments following 7 and 14 days exposure to 30℃. However, the respiratory burst and clearance efficiency of clams fed T. chui and mixture significantly increased from 0.189 and 0％ of unfed treatments to 0.260 ~ 0.287 and to 30％ ~ 34%, respectively for the clams following 14 days exposure to 30℃.
It was concluded that the immune responses of M. lusoria were influenced by the environment temperature. The immune responses of M. lusoria held in 25℃ and 30℃ were higher than clam held in 20℃. The C. muelleri and T. chui have the ability to enhance the activity of immune parameters clams under 25℃and 30℃, and the best immune responses was found in clam fed T. chui. When exposed to different temperature ranged from 20℃ to 30℃, T. chui can enhance the clearance efficiency to the pathogen V. alginolyticus of clam.
| Identifer | oai:union.ndltd.org:TW/095NCYU5613008 |
| Date | January 2007 |
| Creators | Dai-Shiuan Lin, 林岱璇 |
| Contributors | Su-Mei Chen, 陳淑美 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 0 |
Page generated in 0.0082 seconds