碩士 / 國立高雄海洋科技大學 / 水產食品科學研究所 / 95 / Taiwan is a island type nation all sides wreath of the sea, and the ocean is one of the most important native resources for Taiwan. The numerous varieties, huge amount and abundant diversity of bioresources are the unique characteristics of the ocean. This thesis focused on the investigation of the diversity of marine microorganisms in Taiwan and to look for novel bacteria which could produce some valuable bioproducts, and these novel isolates were further subjected to identification and classification. We have isolated 62 strains of halophilic bacteria, including three Gram-positive strains and 59 Gram-negative strains, from seawater samples of the Eluanbi Coast and a shrimp mariculture pond, and samples of a saltpan and the corals.
Phylogenetic analyses based on 16S rRNA gene sequences indicated that these strains belonged to nine genera, such as Alteromonas, Bacillus, Furvibacter, Marinococcus, Oceanicola, Paracoccus, Pseudoalteromonas, Roseobacter and Tenacibaculum. Among these strains, strain AZO-CT, which could accumulate poly-beta-hydroxybutyrate and strain NO.4, which produced ectoine, were chosen for further detailed examinations.
A Gram-negative, aerobic, short rod shaped, poly-beta-hydroxybutyrate granule forming and non-motile bacterial strain, designated AZO-CT, was isolated from the seawater sample collected from the Eluanbi Coast, Pingtung County, southern Taiwan and characterized by a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain formed a monophyletic branch at the periphery of the evolutionary radiation occupied by the genus Oceanicola in the order Rhodobacterales of the Alphaproteobacteria. The closest neighbours were the type strains of Oceanicola batsensis HTCC2597T (95.6 % similarity), Oceanicola nanhaiensis SS011B120T (94.5 %) and Oceanicola granulosus strain HTCC2516T (94.0 %). The fatty acid profile contained predominantly 18:1ω7c (49.1 %) and significant amounts of 19:0 cyclo (24.6 %) and 16:0 (14.7 %). DNA-DNA relatedness of the strain to other species of the genus Oceanicola was less than 70 %. The G+C content of the genomic DNA was 70.9 mol%. The isolate was also distinguished from members of the genus Oceanicola by phenotypic and biochemical characteristics. It is evident from the genotypic, chemotaxonomic and phenotypic data that isolate AZO-CT represents a novel species of the genus Oceanicola, for which the name Oceanicola marinus sp. nov. (type strain AZO-CT= LMG 23705T = BCRC 17591T).
An ectoine-producing bacterium, designated NO.4, was isolated from the saltpan of the Cigu Township, Tainan County, southern Taiwan and characterized by a polyphasic approach. Cells of this isolate were Gram- positive, aerobic, rod shaped, sproe-forming and non-motile. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain formed a monophyletic branch at the periphery of the evolutionary radiation occupied by the genus Marinococcus in the family Sporolactobacillaceae of order Bacillales. The closest neighbours were the type strains of Marinococcus albus DSM 20748T (97.8 % similarity), Marinococcus halophilus DSM 20408T (88.5 %) and Marinococcus halotolerans YIM 70157T (86.3 %). The fatty acid profile contained predominantly ai-C15:0 (40.89 %), i-C15:0 (25.66 %) and ai-C17:0 (13.52 %). The G+C content of the genomic DNA was 51.7 mol%. The isolate was also distinguished from members of the genus Marinococcus by phenotypic and biochemical characteristics. The data from the genotypic, chemotaxonomic and phenotypic analyses suggested that isolate NO.4 seem to be a novel species of the genus Marinococcus.
| Identifer | oai:union.ndltd.org:TW/095NKIM8084002 |
| Date | January 2007 |
| Creators | Kuan-Yin Lin, 林冠穎 |
| Contributors | Wen-Ming Chen, Shih-Yi Sheu, 陳文明, 許世宜 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 135 |
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