碩士 / 國立高雄海洋科技大學 / 水產食品科學研究所 / 95 / Lectins, the carbohydrate glycosylated with peptides or proteins, can bind to other glycans attached to glycoproteins, glycolipids, or polysaccharides with high affinity. Lectins can also bind to the erythrocytes, normal or transformed cell, to cause agglutination. The lectins exist widely in nature, such as in plant, vertebrate and invertebrate. This study is focus to the isolation, characterization of anti-tumor activity by Pleurotus citrinopileatus sing. lectin. The Pleurotus citrinopileatus sing. fruit body was homogenized with distilled water, the crude lectin extract was fractionated at 35-70% saturation of ammonium sulfate, then separated by DEAE-Sepharose anion exchange chromatography, Sephacryl S-200 gel permeation chromatography, electrophoresis and electroelution. After examined the agglutinin reaction with human normal A, B, AB, O type erythrocytes, the results showed that the Pleurotus citrinopileatus sing. lectin exert specifity to O type erythrocytes of human. The molecular weight of Pleurotus citrinopileatus sing. lectin was about 215.7 kDa, which was determined by gel filtration. There were not any effects of some sugars and bivalent cation on the agglutination activity of Pleurotus citrinopileatus sing. lectin. Our studies showed that the Pleurotus citrinopileatus sing. lectin exerted the cytotoxic effect on human tumor cells but not on normal human PBMNCs. By analysis of DNA content using PI and DNA fragmentation in U937 and HL-60 cells, it demonstrated that the Pleurotus citrinopileatus sing. lectin have cytotoxic effect on the leukemia cells by inducing apoptosis.
| Identifer | oai:union.ndltd.org:TW/095NKIM8084004 |
| Date | January 2007 |
| Creators | Hung-Tse Huang, 黃宏澤 |
| Contributors | 江啟銘、陳裕鏞 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 72 |
Page generated in 0.0016 seconds