碩士 / 國立中山大學 / 海洋生物研究所 / 95 / The main objectives of this study is to: (1) understand the recruitment pattern and mechanisms of fish larvae in the kenting waters, such as seasonal variation, distance from shoreline, depth, and diurnal migration of the assemblages of larvae fish. The correlation of the tempo-spatial variations of larval fish communities with temperature, salinity, and chlorophyll were also investigated; (2) to apply DNA barcode technique to identify fish larvae to lower taxonomic categories so as to reveal the temporal or spatial variation of the community structure of fish larvae that was unclear if the identification could only up to higher level. Additionally, DNA barcoding can help us to compile the diagnostic keys for larval fish species identifications.
For fish larval communities studies, fish larvae were collected from four seasons and two different depths during 2001-2002, and every two hours in a day during 10-11 April 2004 by the standard ORI net in Kenting. 527 specimens belonging to 86 taxa and 51 families of fish larvae were collected during 2001-2002. Randomization tests show significant differences between fish larval abundance vs. depths, and the number of families vs. seasons. Canonical correspondence analysis (CCA) shows that temperature and salinity were the main environmental factors, and chlorophyll was the second. 158 specimens belonging to 44 taxa and 34 families of fish larvae were collected during 24 hour survey. The abundance and diversity of fish larvae increase after sunset, and reach to their climax in the midnight.
For COI species identification, 1,505 of the post-flexion stage fish larvae specimens belong to 44 taxa and 34 families were collected by the four-time surveys of light trap in Kenting coral reef area during 2005-2007. Fifteen types of damaselfish larvae and five types of butterflyfish larvae were identified based on their morphological characters. The COI were used to identify the morphological types of damselfishes and butterflyfishes to species level. Fifteen morphological types of damselfishes (Pomacentridae) can be identified to 11 species and three genera. Five morphological types of butterflyfishes (Chaetodontidae) can be assigned to four species and one genus. After COI identification to the lower taxonomic levels, the ordination analysis can clearly reveal the seasonal variation. The result shows that applying DNA barcode technique to identify fish larvae to lower taxonomic categories indeed can reveal temporal or spatial variation of the community structure of fish larvae.
| Identifer | oai:union.ndltd.org:TW/095NSYS5270020 |
| Date | January 2007 |
| Creators | Hui-Ling Ko, 柯慧玲 |
| Contributors | Kwang-Tsao Shao, Li-Lian Liu, 邵廣昭, 劉莉蓮 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 123 |
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