碩士 / 國立臺南大學 / 環境生態研究所碩士班 / 95 / In this study we use on-line solid-phase extraction (on-line SPE) liquid chromatography–tandem mass spectrometry (LC-MS/MS) with isotope-dilution to quantitatively analyze the DNA adducts of N7-methylguanine (N7-MeG) and N7-ethylguanine (N7-EtG) in small fish liver. The results show that N-nitrosodimethylamine (NDMA) induced an apparent linear dose-response for N7-MeG levels in liver tissue and a sublinear dose-response for N7-EtG levels was observed with increasing concentration of N-nitrosodiethylamine (NDEA). When fish received combined treatments of NDMA and NDEA, both N7-MeG and N7-EtG were dose-dependently increased. Moreover, the combined treatment of both agents did not generate significantly more N7-MeG and N7-EtG than either treatment alone. At the time-course experiment, N7-ethylated guanines were removed less efficiently than N7-methylated guanines. In the future, this method could be used at ecological studies to estimate the relationship between DNA alkylation and biological response from exposure to environmental alkylating agents.
| Identifer | oai:union.ndltd.org:TW/095NTNT5587004 |
| Creators | Chia-hua Tan, 譚家驊 |
| Contributors | Mu-Rong Chao, Shyh-Jiun Liu, 趙木榮, 劉世鈞 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 87 |
Page generated in 0.0134 seconds