碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 95 / This paper deals with the effects of saponin and retonone on the enhancement of immune response of white shrimp Litopenaeus vannamei and its resistane against Vibrio alginolyticus when the shrimp were immersed in seawater containing different concentrations of saponin and rotenone.
The haemocyte count, phenoloxidase (PO) activity, specific α2-macroglobulin (α2-M) activity, respiratory burst, superoxide dismutase (SOD) activity, glutathione peroxidase (GPx) activity, phagocytic activity and clearance efficiency against Vibrio alginolyticus were examined when the white shrimp L. vannamei (10.42 ± 2.0 g) were immersed in seawater (34 ‰) containing different concentrations of saponin (0, 0.5, 1 and 2 mg l-1) for 24, 48 and 72 h. The hyaline cell (HC), total haemocyte count (THC), specific α-M activity, respiratory burst, SOD activity, and GPx activity increased directly with saponin, whereas PO activity was inversely related to saponin. L. vannamei that were immersed in saponin at 1 and 2 mg l-1 showed increased phagocytic activity and clearance efficiency to V. alginolyticus over 48-72 h. In another experiment, L. vannamei which had been immersed in seawater containing different concentrations of saponin after 72 h were challenged with V. alginolyticus at 3.2 x 105 colony-forming units (cfu) shrimp-1, and then placed in seawater. The survival of shrimp that immersed in seawater containing saponin at either dose was significantly higher than that of the control shrimp after 12 h, as well as at the termination of the experiment (5 days after the challenge). It was therefore concluded that L. vannamei that were immersed in water containing saponin at 2 mg l-1 or lower exhibited immmunomodulatory effect, as well as protection against V. alginolyticus infection.
The haemocyte count, phenoloxidase (PO) activity, respiratory burst, superoxide dismutase (SOD) activity, glutathione peroxidase (Gpx) activity, phagocytic activity and clearance efficiency against Vibrio alginolyticus were examined when the white shrimp L.vannamei (10.28 ±1.7g) were immersed in seawater (34 ‰) containing different concentrations of rotenone (0,0.5,1 and 2 mg l-1) for 6, 12, 24 and 48 h. The hyaline cell (HC), THC, PO activity, respiratory burst, SOD activity, and GPx activity increased directly with rotenone. L. vannamei that were immersed in retenone at 1 and 2 mg l-1 showed increased phagocytic activity and clearance efficiency to V. alginolyticus after 12 h. In another experiment, L. vannamei which had been immersed in seawater containing different concentarations of rotenone after 24 h were challenged with V. alginolyticus at 4.0 x 105 colony-forming units (cfu) shrimp-1, and then placed in seawater. The survival of shrimp that immersed in seawater containing rotenone at 1 and 2 mg l-1 was significantly higher than that of control shrimp after 6~12 h, as well as at the termination of the experiment (3 days after the challenge). It was therefore concluded that L. vannamei that were immersed in seawater containing rotenone at 2 mg l-1 or lower exhibited immunomdulatory effect, as well as protection against V.alginolyticus infection.
Keyword：Litopenaeus vannamei ; Vibrio alginolyticus; Saponin ; Rotenone;Immersion ; Haemocyte count ; Phenoloxidase activity;Specific α2-macroglobulin; Respiratory burst; Superoxide dismutase activity; Glutathione peroxidase activity; Phagocytic activity; Clearance efficiency; Resistance.
| Identifer | oai:union.ndltd.org:TW/095NTOU5086029 |
| Date | January 2007 |
| Creators | BO-Kun Su, 蘇博堃 |
| Contributors | Jiann-Chu Chen, 陳建初 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 71 |
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