碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 95 / Abstract
Grey mullet, Mugil cephalus is an important commercial species cultured in Taiwan. Due to their valuable ovary product cultured grey mullet can make more profits than other fish species. There are five grey mullet strains found in Taiwan. The superior quality of the ovary product was obtained in some strains and the poor ovary maturation will impede in this industry. However, it has been difficult to distinguish from their ovary size and gonadosomatic index (GSI). Therefore, the microsatellite DNA markers were used to examine those different strains. In this study, Ten polymorphic microstatellites were used in this study. The DNA markers were applied to evaluate the genetic structure of different strains. The microsatellite markers were used to test 14 individuals from two places. The numbers of alleles were from 4~15, respectively. High levels of allelic and genetic diversity were observed in Mcs 6 loci. The polymorphic microsatellites are useful in monitoring genetic diversity of cultured grey mullets.
Mitochondrial DNA was used to distinguish the grey mullet fry obtained from the different regions in Taiwan. Specific amplification primers for 12S-rDNA and cytochrome b were designed and used to characterize the species origin. In addition to differentiating grey mullet from Liza carinata, this method is also sensitive to distinguish grey mullet fry from different region.
| Identifer | oai:union.ndltd.org:TW/095NTOU5086035 |
| Date | January 2007 |
| Creators | Jian Ya-Huei, 簡雅蕙 |
| Contributors | Lin Cheng-Hui, 林正輝 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 45 |
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