碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 95 / Abstract
The objectives of the study were to investigate the effects of different salinities and chemical stimulus in CRH, CRHR, HSP70, IL-1β and Na+/K+-ATPase gene expressions in vivo and in vitro gill of black porgy (Acanthopagrus schlegeli ). In vivo experiment, CRH, CRHR, HSP70, IL-1β and Na+/K+-ATPase gene expressions were compared in different tissues. In vitro gill culture, CRH had more effects in the gill expression of genes than dexamethasone. The results showed that CRH and CRHR increased in high dosage CRH. In freshwater and seawater gill culture experiment in a short period( 4 ~ 8 hours), CRH, CRHR, HSP70, IL-1β and Na+/K+-ATPase genes had conspicuous changes in the gill. It is indicated that in vitro gill could monitor the salinity changes. In long term and short term salinity change experiments, the gene expressions in freshwater group were higher than in seawater group. Only CRHR expression decreased in short term freshwater acclimation. Na+/K+-ATPase transcripts had significantly changes in gill, but no difference in midbrin, hypothalamus and pituitary. Gill structure was stained by hematoxylin-eosin. Cells related to Na+/K+-ATPase and CRH expression were further identified in the gill epithelium by immunohistochemiscal stain. The results show that CRH certainly expresses in the gill and gill is an organ to respond the stress status.
| Identifer | oai:union.ndltd.org:TW/095NTOU5086055 |
| Date | January 2007 |
| Creators | Jyun-Cing Cao, 曹俊清 |
| Contributors | Ching-Fong Chang, 張清風 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 89 |
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