碩士 / 國立臺灣海洋大學 / 食品科學系 / 95 / Astaxanthin (AST), a non-pro-vitamin A carotenoid, is abundant in aquatic animals such as crab, shrimp etc. It possesses some pharmacological activities, including anti-inflammatory, anti-oxidative and immunomodulatory properties. IgE-mediated allergy owing to hyper-reactive immune responses to antigen in the environment. It shows high relationship beteewn reactive oxygen species (ROS) and allergic response through modulating intracellular signal transduction pathway. These actions cause the process of degranulation of mast cells resulting in the release of allergic mediators. In this report, we investigate the anti-allergic effect of AST and its possible mechanisms. The allergic model is established by RBL-2H3 and KU812 cells that sensitized by antigen and calcium ionophore (A23187). The results prove 100 uM AST inhibits 30 % of intracellular Ca+2 influx and almost abolished protein kinase C (PKC) phosphorylation and 40 % of ROS generation. Treating with 10-100 uM AST inhibits 20 % of histamine, b-hexosaminidase release. Expression the receptor for IgE (FceRI) is only inhibited by treating with high concentration (300 uM) AST. AST significantly reduce of IL-4 release at 100 uM and the IC50 for mitogen-activated protein kinases (MAPK) at 30 uM.
And all above, these findings elucidate that astaxanthin can alleviate the IgE-mediate allergic response major by down-regulating ROS generation, reducing intracellular signaling pathways (Ca+2 influx, PKC and MAPK) and chemical mediators (histamine, b-hexosaminidase and IL-4) release.
Key words: Astaxanthin, IgE-mediated allergic responses, mast cell, degranulation, basophil
| Identifer | oai:union.ndltd.org:TW/095NTOU5253017 |
| Date | January 2007 |
| Creators | Hsing-Wen Hung, 洪幸彣 |
| Contributors | 龔瑞林 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 85 |
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